4YAA
YopH W354Y Yersinia enterocolitica PTPase apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-3 |
| Synchrotron site | SSRL |
| Beamline | BL9-3 |
| Temperature [K] | 194 |
| Detector technology | CCD |
| Collection date | 2012-02-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.888, 60.512, 89.358 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.130 - 1.050 |
| R-factor | 0.129 |
| Rwork | 0.128 |
| R-free | 0.14000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f99 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.317 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.036 | 0.414 |
| Number of reflections | 133901 | |
| <I/σ(I)> | 48.3 | 4.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 8.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | peg 3350, HEPES |
