4YA6

Crystal structure of LigO-apo form from Sphingobium sp. strain SYK-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99999
Spacegroup name	P 32 2 1
Unit cell lengths	79.229, 79.229, 72.185
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.799
R-factor	0.18
Rwork	0.178
R-free	0.20640
RMSD bond length	0.003
RMSD bond angle	0.773
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.790
Number of reflections	24752
<I/σ(I)>	46.3
Completeness [%]	100.0
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Ammonium Citrate, 0.1 M MES pH 5.5, 20% PEG 3,350, 5% Isopropanol