4Y9V
Gp54 tailspike of Acinetobacter baumannii bacteriophage AP22 in complex with A. baumannii capsular saccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 92.634, 92.634, 391.478 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.900 - 0.900 |
| R-factor | 0.0972 |
| Rwork | 0.097 |
| R-free | 0.10920 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 0.960 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.073 | 0.353 |
| Number of reflections | 466729 | |
| <I/σ(I)> | 13.7 | 3 |
| Completeness [%] | 97.7 | 86.3 |
| Redundancy | 4.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 6-10% PEG8000, 500mM Li2SO4, 100mM MES pH 6.5 |
