4Y9L

Crystal Structure of Caenorhabditis elegans ACDH-11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-21
Detector	RAYONIX MX-225
Wavelength(s)	0.99985
Spacegroup name	C 1 2 1
Unit cell lengths	137.925, 113.396, 113.901
Unit cell angles	90.00, 123.98, 90.00

Refinement procedure

Resolution	50.000 - 2.270
R-factor	0.1698
Rwork	0.167
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3djl
RMSD bond length	0.017
RMSD bond angle	1.817
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.310
High resolution limit [Å]	2.270	2.270
Rmerge	0.110	0.394
Number of reflections	66307
<I/σ(I)>	11.6	4.1
Completeness [%]	98.9	83.6
Redundancy	7.2	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	PEG 3350, Tris, magnesium formate