4Y9A
Crystal structure of Triosephosphate Isomerase from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 86.069, 86.069, 133.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.634 - 2.294 |
| R-factor | 0.2315 |
| Rwork | 0.229 |
| R-free | 0.28180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ta6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.334 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.030 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.071 | 0.371 |
| Number of reflections | 43475 | |
| <I/σ(I)> | 10 | 3 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1 M TRIS hydrochloride pH 8.5, 2.0 M ammonium sulfate |
