4Y91

Crystal Structure of a Thermotoga maritima Hfq homolog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97879
Spacegroup name	P 21 21 21
Unit cell lengths	39.080, 133.500, 206.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.030 - 2.656
R-factor	0.1868
Rwork	0.184
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hsb
RMSD bond length	0.012
RMSD bond angle	1.479
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.030		2.720
High resolution limit [Å]	2.656	11.880	2.660
Rmerge	0.119	0.029	0.616
Rmeas	0.123	0.030	0.643
Total number of observations	393986
Number of reflections	27360	438	641
<I/σ(I)>	25.39	62.97	5.85
Completeness [%]	84.7	99.3	27.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	tri-potassium citrate, PEG-3350