4Y8F
Crystal structure of Triosephosphate Isomerase from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-02-29 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.255, 49.555, 71.502 |
Unit cell angles | 90.00, 119.11, 90.00 |
Refinement procedure
Resolution | 37.589 - 1.540 |
R-factor | 0.1484 |
Rwork | 0.147 |
R-free | 0.18130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1btm |
RMSD bond length | 0.010 |
RMSD bond angle | 1.239 |
Data reduction software | CrystalClear |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1313)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.589 | 1.620 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.062 | 0.448 |
Number of reflections | 33930 | |
<I/σ(I)> | 14.6 | 3.1 |
Completeness [%] | 99.2 | 94.4 |
Redundancy | 4.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291.15 | 0.2 M ammonium acetate, 0.1 M sodium acetate pH 4.6, 30% w/v polyethylene glycol (PEG) 4000 |