4Y88
Crystal structure of the N-terminal domain of CEACAM8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 94.336, 94.336, 94.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.710 - 1.450 |
| R-factor | 0.1467 |
| Rwork | 0.145 |
| R-free | 0.17980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qsq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.505 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.710 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 26074 | |
| <I/σ(I)> | 27 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 48.9 | 49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 1.2M Ammmonium Sulphate, 0.1M Citric Acid, pH 3.5 |
