4Y7S
Crystal Structure of the CFEM protein Csa2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-12-02 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.341, 61.208, 97.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.672 - 2.000 |
R-factor | 0.1545 |
Rwork | 0.153 |
R-free | 0.19160 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.528 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.420 | 2.000 |
Rmerge | 0.061 | 0.027 | 0.625 |
Rmeas | 0.063 | 0.028 | 0.688 |
Rpim | 0.016 | 0.007 | 0.275 |
Total number of observations | 370056 | ||
Number of reflections | 25528 | ||
<I/σ(I)> | 12.7 | 2.04 | |
Completeness [%] | 96.0 | 99.3 | 67.5 |
Redundancy | 15.1 | 15.5 | 5.4 |
CC(1/2) | 1.000 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Protein was mixed at ration of 2:1 with a reservoir (0.1M HEPES pH 7.5, 8% EG, 13.75% PEG 10,000) |