4Y7O
T6SS protein TssM C-terminal domain (869-1107) from EAEC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.470, 85.470, 256.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.340 - 2.240 |
| R-factor | 0.2047 |
| Rwork | 0.204 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y7l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.067 | 0.730 |
| Number of reflections | 46408 | |
| <I/σ(I)> | 21.5 | 3.1 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 10 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.75 | 293 | 0.05M ZnCl2, 15% PEG6000 and 0.1 NaAc pH 4.75. |
