4Y7M
T6SS protein TssM C-terminal domain (835-1129) from EAEC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 64 |
| Unit cell lengths | 95.230, 95.230, 172.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.610 - 1.920 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qgy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.910 |
| Rmerge | 0.079 | 1.000 |
| Number of reflections | 67557 | |
| <I/σ(I)> | 18 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18-20 % PEG4000, 0.25 M Ammonium sulfate. |
