4Y7L
T6SS protein TssM C-terminal domain (869-1107) from EAEC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.030, 64.030, 249.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.110 - 1.510 |
| R-factor | 0.1919 |
| Rwork | 0.191 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y7m |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.067 | 0.590 |
| Number of reflections | 82164 | |
| <I/σ(I)> | 19.2 | 3 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 9.9 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350, 0.1M NaAc pH 4.0 and 0.2M ZnCl2. |
