4Y7K
Structure of an archaeal mechanosensitive channel in closed state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.292, 140.368, 182.544 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.000 - 3.500 |
R-factor | 0.26085 |
Rwork | 0.259 |
R-free | 0.28709 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oar 2b98 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.568 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 1.000 | |
Number of reflections | 27942 | |
<I/σ(I)> | 26.2 | 1.8 |
Completeness [%] | 99.2 | 100 |
Redundancy | 11 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 289 | 7% PEG4000, 0.4M NH4SCN, 0.1M Citric acid(pH7.3) |