4Y7J
Structure of an archaeal mechanosensitive channel in expanded state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 147.360, 149.250, 99.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 4.100 |
R-factor | 0.32335 |
Rwork | 0.321 |
R-free | 0.37582 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b98 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.116 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 4.200 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmerge | 0.109 | 0.938 |
Number of reflections | 17722 | |
<I/σ(I)> | 8.7 | 2.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 9 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 1.6M (NH4)2SO4, 0.1M NaCl, 0.1M HEPES |