4Y7C
rat CYPOR mutant - G141del/E142N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-12-19 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.485, 115.643, 118.882 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.540 - 2.200 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1amo |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.540 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.076 |
Number of reflections | 68829 |
<I/σ(I)> | 26 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | PEG 4000, HEPES, NaAc |