4Y6T
Structure of Tobacco streak virus coat protein dimer at 2.4 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.040, 69.980, 103.440 |
| Unit cell angles | 90.00, 105.51, 90.00 |
Refinement procedure
| Resolution | 99.670 - 2.400 |
| R-factor | 0.2093 |
| Rwork | 0.207 |
| R-free | 0.24430 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.724 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.673 | 40.340 | 2.530 |
| High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
| Rmerge | 0.034 | 0.692 | |
| Rmeas | 0.101 | ||
| Rpim | 0.036 | 0.013 | 0.264 |
| Total number of observations | 422296 | 13312 | 61035 |
| Number of reflections | 54445 | ||
| <I/σ(I)> | 16.3 | 40.7 | 3.2 |
| Completeness [%] | 97.8 | 97.9 | 97.2 |
| Redundancy | 7.8 | 7.3 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 12% PEG 8000, 10% glycerol, 0.5M potassium chloride |
