4Y6C

Q17M crystal structure of Podosopora anserina putative kinesin light chain nearly identical TPR-like repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	80
Detector technology	CCD
Collection date	2013-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	81.103, 91.242, 30.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.760 - 1.772
R-factor	0.175
Rwork	0.173
R-free	0.21350
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.922
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.760	1.836
High resolution limit [Å]	1.772	1.773
Rmerge	0.175	0.348
Number of reflections	23013
<I/σ(I)>	17.7	11.4
Completeness [%]	100.0	99.48
Redundancy	14.3	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295.16	0.1 M MES, 35% (w/v) PEG 4000