4Y61
Crystal structure of the complex between Slitrk2 LRR1 and PTP delta Ig1-Fn1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.227, 91.308, 123.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.654 - 3.358 |
| R-factor | 0.2373 |
| Rwork | 0.235 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd6 2yd9 2dju and 1OZN |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.410 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.407 | |
| Number of reflections | 14509 | |
| <I/σ(I)> | 14.3 | 1.9 |
| Completeness [%] | 99.2 | 97.2 |
| Redundancy | 9.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 15% PEG4000, 0.1 M sodium acetate, 0.1 M MES |
