4Y60

Structure of SOX18-HMG/PROX1-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-15
Detector	ADSC QUANTUM 1
Wavelength(s)	1.075
Spacegroup name	P 64
Unit cell lengths	111.942, 111.942, 32.417
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.642 - 1.750
R-factor	0.175
Rwork	0.174
R-free	0.19890
RMSD bond length	0.010
RMSD bond angle	1.904
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.750
Number of reflections	23871
<I/σ(I)>	19.94
Completeness [%]	100.0
Redundancy	24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	0.1M MMT buffer pH 5.0, 25% (w/v) PEG 1500