4Y5W
Transcription factor-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 68.298, 94.241, 147.785 |
Unit cell angles | 99.85, 101.73, 89.93 |
Refinement procedure
Resolution | 38.807 - 3.104 |
R-factor | 0.2511 |
Rwork | 0.250 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y5u |
RMSD bond length | 0.008 |
RMSD bond angle | 1.064 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 57478 | |
<I/σ(I)> | 11.2 | 1.65 |
Completeness [%] | 98.0 | 95.2 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M citrate (pH 5.6), 0.1M NaCl, 20% isopropyl alcohol and 8% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |