4Y59
Crystal structure of ALiS1-Streptavidin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.843, 84.353, 46.156 |
| Unit cell angles | 90.00, 98.92, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.220 |
| R-factor | 0.12615 |
| Rwork | 0.125 |
| R-free | 0.15545 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.343 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.069 | 0.380 |
| Number of reflections | 129491 | |
| <I/σ(I)> | 10.3 | 3.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Hepes-NaOH (pH7.0), 50%(v/v) MPD |
