4Y52

Crystal structure of 5-Carboxycytosine Recognition by RNA Polymerase II during Transcription Elongation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	166.703, 221.644, 192.410
Unit cell angles	90.00, 100.35, 90.00

Refinement procedure

Resolution	49.273 - 3.500
R-factor	0.2027
Rwork	0.201
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m3y
RMSD bond length	0.009
RMSD bond angle	1.355
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.560
High resolution limit [Å]	3.500	9.480	3.500
Rmerge	0.143	0.038	0.583
Total number of observations	296275
Number of reflections	81682
<I/σ(I)>	5.1
Completeness [%]	94.3	100	72.8
Redundancy	3.6	3.8	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	390 mM (NH4)2HPO4/NaH2PO4, 50 mM dioxane, 10 mM DTT, and 10.7-11.6%(w/v) PEG6000