4Y4R
Crystal structure of ribosomal oxygenase NO66 dimer mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.060, 144.210, 144.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.21973 |
| Rwork | 0.217 |
| R-free | 0.27096 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.620 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.580 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.101 | 0.613 |
| Number of reflections | 27284 | |
| <I/σ(I)> | 12.4 | 3 |
| Completeness [%] | 98.8 | 98 |
| Redundancy | 6.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1.0M ammonium monohydric phosphate, 0.1M acetate pH4.5 |
