4Y3I
PAS-GAF fragment from Deinococcus radiodurans BphP assembled with BV - Y307S, low dose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.481, 51.917, 80.339 |
| Unit cell angles | 90.00, 116.22, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.690 |
| R-factor | 0.19206 |
| Rwork | 0.190 |
| R-free | 0.22614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 209C |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.363 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.738 |
| High resolution limit [Å] | 1.690 | 1.694 |
| Rmerge | 0.283 | |
| Number of reflections | 34408 | |
| <I/σ(I)> | 21.4 | 3.8 |
| Completeness [%] | 97.9 | 80.82 |
| Redundancy | 5.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 296 | 19% isopropanol, 19% PEG3350, 5% glycerol, 100 mM citric acid/NaOH |
