4Y31

Crystal structure of yellow lupine LlPR-10.1A protein in ligand-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	35.065, 56.501, 61.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.270 - 1.320
R-factor	0.15296
Rwork	0.151
R-free	0.21152
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1icx
RMSD bond length	0.019
RMSD bond angle	1.818
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.059
Number of reflections	29618
<I/σ(I)>	19.7	2
Completeness [%]	99.9	99.7
Redundancy	3.7	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	18% PEG4000, 0.2 M sodium acetate, 0.1 M HEPES