4Y2K
reduced form of apo-GolB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 37.008, 37.008, 169.938 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.490 - 1.700 |
| R-factor | 0.20634 |
| Rwork | 0.205 |
| R-free | 0.23255 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.110 | |
| Number of reflections | 8190 | |
| <I/σ(I)> | 28.9 | 7.52 |
| Completeness [%] | 97.2 | 92.4 |
| Redundancy | 6.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.0 M Ammonium citrate tribasic, 0.1 M BIS-TRIS propane |
