4Y2F
CRYSTAL STRUCTURE OF NATIVE GAF DOMAIN of POTASSIUM SENSOR HISTIDINE KINASE KDPD FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 35.250, 57.540, 61.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.058 - 1.396 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.886 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev-2313_1839: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.770 | 1.446 |
| High resolution limit [Å] | 1.390 | 1.396 |
| Number of reflections | 24876 | |
| <I/σ(I)> | 18 | |
| Completeness [%] | 98.3 | 97.1 |
| Redundancy | 14.1 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30-35% PEG 3350, 0.1 M Bis-TRIS pH 6.0 - 6.5 |
