4Y27

E.coli 23S Sarcin-Ricil Loop, modified with a 2-Me on G2661 and a methylphosphonate on A2662

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.77491
Spacegroup name	P 43
Unit cell lengths	29.500, 29.500, 77.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.500 - 0.998
R-factor	0.1361
Rwork	0.135
R-free	0.14880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.016
RMSD bond angle	1.579
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.020
High resolution limit [Å]	0.998	0.998
Rmerge	0.058	3.697
Number of reflections	35632
<I/σ(I)>	35.15	1.01
Completeness [%]	99.8	98
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Crystals were grown for two weeks at 20C by mixing one volume of RNA sample with one volume of a crystallization buffer made with ammonium sulfate 2.5 M, magnesium acetate 10 mM and 2-(N-morpholino)ethanesulfonic acid (MES) pH 5.6 50 mM.