4Y26

Complex of human Galectin-7 and Galbeta1-3(6OSO3)GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97622
Spacegroup name	P 21 21 21
Unit cell lengths	30.098, 55.404, 138.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.613 - 2.611
R-factor	0.2002
Rwork	0.198
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.671
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.3)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.700
High resolution limit [Å]	2.610	5.610	2.610
Rmerge	0.118	0.073	0.438
Total number of observations	42427
Number of reflections	7526
<I/σ(I)>	7.3
Completeness [%]	99.0	97.2	100
Redundancy	5.6	4.9	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M HEPES (pH 7.5), 0.2M Li2SO4, 0.1M NaOAc, 25% (w/v) PEG 4000