4Y23
Crystal structure of T399A precursor mutant protein of gamma-glutamyl transpeptidase from Bacillus licheniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-10 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.438, 59.438, 281.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.555 - 2.890 |
| R-factor | 0.2241 |
| Rwork | 0.221 |
| R-free | 0.27550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ott |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.703 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.119 | 0.451 |
| Number of reflections | 13299 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 96.2 | 97.9 |
| Redundancy | 4.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 45% MPD, 0.1 M Bis-Tris pH 6.5 and 0.2 M ammonium acetate |
