4Y1X
Complex of human Galectin-1 and Galbeta1-4(6OSO3)GlcNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.579, 57.220, 110.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.293 - 2.450 |
| R-factor | 0.2145 |
| Rwork | 0.210 |
| R-free | 0.25900 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 5.270 | 2.450 |
| Rmerge | 0.068 | 0.042 | 0.496 |
| Total number of observations | 54812 | ||
| Number of reflections | 10845 | ||
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 99.7 | 98.5 | 99.6 |
| Redundancy | 5.1 | 4.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1 M Tris (pH 8.0), 0.2 M Li2SO4, 30% (w/v) PEG 3350 |
