4Y1W

Vis toxin, an ADP-ribosyltransferase from Vibrio Splendidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-18
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	48.500, 51.800, 102.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.404 - 1.400
R-factor	0.1564
Rwork	0.155
R-free	0.18480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.226
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.404	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.064
Number of reflections	50597
<I/σ(I)>	14.73
Completeness [%]	97.7	83.48
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	297.15	30% Jeffamine ED-2001, 0.1 M Hepes