4Y1V

Complex of human Galectin-1 and Galbeta1-3GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97622
Spacegroup name	P 21 21 21
Unit cell lengths	43.715, 58.291, 111.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.833 - 2.320
R-factor	0.222
Rwork	0.217
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w6n
RMSD bond length	0.006
RMSD bond angle	0.971
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.3)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.400
High resolution limit [Å]	2.320	4.990	2.320
Rmerge	0.041	0.027	0.208
Rmeas	0.047	0.031	0.243
Rpim	0.022	0.015	0.123
Total number of observations	57534
Number of reflections	12886
<I/σ(I)>	18.2
Completeness [%]	99.2	98.8	95.2
Redundancy	4.5	4.1	3.8
CC(1/2)		0.998	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1 M Tris (pH 8.0), 0.2 M Li2SO4, 30% (w/v) PEG 3350