4Y1Q
Crystal Structure of HasA mutant Y75A monomer from Yersinia pseudotuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-22 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 69.660, 70.320, 77.030 |
Unit cell angles | 89.35, 89.75, 90.46 |
Refinement procedure
Resolution | 41.846 - 3.100 |
R-factor | 0.2224 |
Rwork | 0.220 |
R-free | 0.27560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xzd |
RMSD bond length | 0.003 |
RMSD bond angle | 0.817 |
Data reduction software | SCALA (3.3.21) |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.686 | 49.686 | 3.270 |
High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
Rmerge | 0.033 | 0.395 | |
Rmeas | 0.193 | ||
Rpim | 0.137 | 0.033 | 0.395 |
Total number of observations | 49786 | 1572 | 7241 |
Number of reflections | 25512 | ||
<I/σ(I)> | 5.7 | 16.1 | 2 |
Completeness [%] | 96.1 | 95.8 | 95.7 |
Redundancy | 2 | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 1 M Bicine, 1.8 M Ammnonium acetate |