4Y1O

Oceanobacillus iheyensis group II intron domain 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	84.345, 132.250, 84.561
Unit cell angles	90.00, 91.87, 90.00

Refinement procedure

Resolution	40.250 - 2.950
R-factor	0.1811
Rwork	0.179
R-free	0.22380
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	model from an isomorphous crystal
RMSD bond length	0.005
RMSD bond angle	1.099
Data reduction software	XDS (January 10, 2014)
Data scaling software	XDS (January 10, 2014)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.250	3.060
High resolution limit [Å]	2.950	2.950
Number of reflections	75788
<I/σ(I)>	19.31	1.65
Completeness [%]	98.7	94.95
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298.15	80 mM NaCl, 24 mM KCl, 5 mM MgCl2, 40 mM NaCacodylate, 17% MPD, 8 mM spermine-4HCl