4Y0V
Structure of an ADP ribosylation factor from Entamoeba histolytica HM-1:IMSS bound to Mg-GDP
Replaces: 3RD1Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.540, 40.660, 78.950 |
Unit cell angles | 90.00, 97.45, 90.00 |
Refinement procedure
Resolution | 19.729 - 1.800 |
R-factor | 0.2252 |
Rwork | 0.223 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r8s |
RMSD bond length | 0.007 |
RMSD bond angle | 1.127 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1932)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.729 | 1.850 | |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.060 | 0.022 | 0.431 |
Rmeas | 0.069 | 0.026 | 0.486 |
Total number of observations | 141065 | ||
Number of reflections | 34091 | 406 | 2600 |
<I/σ(I)> | 15.97 | 38.05 | 3.24 |
Completeness [%] | 93.4 | 90 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 15% ethanol, 0.1M Tris, Cryoprotection 25% Ethylene glycol, pH 7.0 |