4Y0T
Crystal structure of apo form of OXA-58, a Carbapenem hydrolyzing Class D beta-lactamase from Acinetobacter baumanii (P21, 4mol/ASU)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.017, 65.095, 191.964 |
| Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
| Resolution | 34.790 - 2.300 |
| R-factor | 0.19651 |
| Rwork | 0.194 |
| R-free | 0.24328 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.675 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.790 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.120 | 0.420 |
| Number of reflections | 37470 | |
| <I/σ(I)> | 9.15 | |
| Completeness [%] | 91.2 | 74.8 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris base/Hydrochloric acid (pH 8.0) and 28% (w/v) PEG 3350 |
