4Y0S
Goat beta-lactoglobulin complex with pramocaine (GLG-PRM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2014-05-20 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 53.113, 53.113, 111.148 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 13.070 - 1.900 |
R-factor | 0.21854 |
Rwork | 0.213 |
R-free | 0.26532 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bsy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.338 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.070 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.081 | 0.224 |
Number of reflections | 14765 | |
<I/σ(I)> | 6.6 | 2.3 |
Completeness [%] | 99.3 | 99 |
Redundancy | 2.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | protein 10 mg/ml, 2.30 M (NH4)2SO4 in 0.5 Tris-HCl pH 8.5, 5 mM pramociane |