4Y0S
Goat beta-lactoglobulin complex with pramocaine (GLG-PRM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2014-05-20 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.113, 53.113, 111.148 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.070 - 1.900 |
| R-factor | 0.21854 |
| Rwork | 0.213 |
| R-free | 0.26532 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bsy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.338 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.070 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.224 |
| Number of reflections | 14765 | |
| <I/σ(I)> | 6.6 | 2.3 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | protein 10 mg/ml, 2.30 M (NH4)2SO4 in 0.5 Tris-HCl pH 8.5, 5 mM pramociane |
