4Y0R
Bovine beta-lactoglobulin complex with pramocaine crystallized from ammonium sulphate (BLG-PRM2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2013-04-29 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.735, 53.735, 110.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.430 - 2.300 |
| R-factor | 0.22128 |
| Rwork | 0.212 |
| R-free | 0.28549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bsy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.495 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.430 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.055 | 0.162 |
| Number of reflections | 8567 | |
| <I/σ(I)> | 10.4 | 2.6 |
| Completeness [%] | 98.9 | 96.6 |
| Redundancy | 2.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | protein 20 mg/ml, 2.4 M (NH4)2SO4 in 0.5 M Tris-HCl pH 8.9 and 5 mM pramociane |
