4Y0Q
Bovine beta-lactoglobulin complex with pramocaine crystallized from sodium citrate (BLG-PRM1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2012-04-23 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.525, 53.525, 110.959 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.290 - 2.000 |
| R-factor | 0.22232 |
| Rwork | 0.216 |
| R-free | 0.27843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bsy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.351 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.290 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.344 |
| Number of reflections | 12846 | |
| <I/σ(I)> | 10 | 1.9 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 2.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | protein 20 mg/ml, 1.34 M sodium citrate in 0.1 M Tris-HCl pH 7.5 |
