4Y0G
beta2 carbohydrate binding module (CBM) of AMP-activated protein kinase (AMPK)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-20 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.953700 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.973, 27.627, 78.882 |
Unit cell angles | 90.00, 133.88, 90.00 |
Refinement procedure
Resolution | 40.720 - 1.600 |
R-factor | 0.15154 |
Rwork | 0.150 |
R-free | 0.18247 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.889 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.720 | 1.620 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.669 | |
Number of reflections | 23221 | |
<I/σ(I)> | 17.8 | 2.8 |
Completeness [%] | 97.6 | 90.7 |
Redundancy | 7.5 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281.15 | 0.17 M ammonium sulphate, 15% v/v glycerol and 25.5 % w/v PEG4000 |