4Y0C
The structure of Arabidopsis ClpT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-02-07 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.150, 57.770, 61.650 |
| Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
| Resolution | 25.396 - 1.992 |
| R-factor | 0.1756 |
| Rwork | 0.173 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fh2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.134 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.080 |
| High resolution limit [Å] | 1.992 | 1.992 |
| Rmerge | 0.413 | |
| Number of reflections | 23802 | |
| <I/σ(I)> | 21.6 | 3.6 |
| Completeness [%] | 95.4 | 68 |
| Redundancy | 4.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6 M ammonium sulphate, 10% dioxane, 0.1 M MES |
