4Y01

Crystal structure of dipeptidyl peptidase 11 (DPP11) from Porphyromonas gingivalis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-12-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	99.788, 115.486, 147.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.460
R-factor	0.1866
Rwork	0.184
R-free	0.23339
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.757
Phasing software	SHARP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.520
High resolution limit [Å]	2.460	2.460
Number of reflections	62234
<I/σ(I)>	12.6	2.1
Completeness [%]	99.6	99.5
Redundancy	6.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	BICINE pH 9.0, sodium chloride, PEG MME 550