4XZW
Endo-glucanase chimera C10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.251, 83.248, 121.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.1701 |
| Rwork | 0.169 |
| R-free | 0.19998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xzb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.337 |
| Data reduction software | Coot |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 51327 |
| <I/σ(I)> | 32.5 |
| Completeness [%] | 99.5 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 291 | 25 mM 18-crown-6, 30% (w/v) PEG 4000, 0.1 M HEPES sodium salt, pH 7.4, 0.2 M calcium chloride. |
