4XZC
The crystal structure of Kupe virus nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.981, 73.981, 423.978 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.059 - 2.601 |
R-factor | 0.2153 |
Rwork | 0.212 |
R-free | 0.28040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u3i |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.422 | |
Number of reflections | 37285 | |
<I/σ(I)> | 20.92 | 4.296 |
Completeness [%] | 98.5 | 93.9 |
Redundancy | 11.3 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 200mM sodium nitrate (PH 8.0), 5mM cobalt chloride, 8% (wt/vol) PEG 3350 |