4XZ8
The crystal structure of Erve virus nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.899, 67.895, 154.079 |
Unit cell angles | 90.00, 93.56, 90.00 |
Refinement procedure
Resolution | 33.454 - 2.350 |
R-factor | 0.1775 |
Rwork | 0.174 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u3i |
RMSD bond length | 0.008 |
RMSD bond angle | 1.109 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.350 |
Number of reflections | 42902 |
<I/σ(I)> | 9.071 |
Completeness [%] | 99.3 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 200mM sodium malonate, 20% (wt/vol) PEG 3350,7% (vol/vol) 1-Butanol |