4XYQ
Structure of AgrA LytTR domain in complex with promoters
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-24 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.770, 47.770, 100.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.770 - 2.400 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23752 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.537 |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.120 | 2.490 |
| High resolution limit [Å] | 2.380 | 2.400 |
| Rmerge | 0.461 | |
| Number of reflections | 8820 | |
| <I/σ(I)> | 9.6 | 2.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M Bis-Tris pH 6.0, 40% PEG 400. |
