Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.785, 86.684, 66.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.882 - 2.550 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.500 |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.882 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 12927 | |
| <I/σ(I)> | 12.9 | 2.2 |
| Completeness [%] | 95.7 | 98.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 0.1M Na cacodylate, pH 6.8, 25% w/v PEG 3350, 9mM CaCl2 |
