4XXF
L-fuculose 1-phosphate aldolase from Glaciozyma antarctica PI12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 84.330, 84.330, 78.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.710 - 1.340 |
R-factor | 0.1722 |
Rwork | 0.171 |
R-free | 0.18700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ocr |
RMSD bond length | 0.006 |
RMSD bond angle | 1.063 |
Data reduction software | Coot |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.710 | 1.410 |
High resolution limit [Å] | 1.340 | 1.340 |
Rmerge | 0.104 | |
Number of reflections | 61957 | |
<I/σ(I)> | 14.6 | 2 |
Completeness [%] | 96.9 | 81.9 |
Redundancy | 15.7 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | PEG 8000, magnesium chloride, Tris-HCl |