4XX5
Structure of PI3K gamma in complex with an inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2009-04-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.444, 67.418, 106.698 |
Unit cell angles | 90.00, 95.67, 90.00 |
Refinement procedure
Resolution | 106.180 - 2.760 |
R-factor | 0.202 |
Rwork | 0.198 |
R-free | 0.27420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.476 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 106.180 |
High resolution limit [Å] | 2.760 |
Number of reflections | 18263 |
<I/σ(I)> | 5.1 |
Completeness [%] | 72.5 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% PEG3350, 200 MM LITHIUM SULFATE,10 MM DTT, 10 MM EDTA, 100 MM TRIS |