4XX5
Structure of PI3K gamma in complex with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.444, 67.418, 106.698 |
| Unit cell angles | 90.00, 95.67, 90.00 |
Refinement procedure
| Resolution | 106.180 - 2.760 |
| R-factor | 0.202 |
| Rwork | 0.198 |
| R-free | 0.27420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.476 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 106.180 |
| High resolution limit [Å] | 2.760 |
| Number of reflections | 18263 |
| <I/σ(I)> | 5.1 |
| Completeness [%] | 72.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% PEG3350, 200 MM LITHIUM SULFATE,10 MM DTT, 10 MM EDTA, 100 MM TRIS |
